酶切

网络BamHI; Restriction enzyme cutting; off-target

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1.	BamHI ...两条路线——双酶切(HindⅢEcoRI)路线和单酶切(BamHI)路线,以BL21(DE3)为宿主菌构建了两种胞内表达AspAT的基因工程 … cpfd.cnki.com.cn\|基于17个网页
2.	Restriction enzyme cutting 3.2.4 酶切 (Restriction enzyme cutting) 43 3.5.5 凝胶电泳 (Gel electrophoresis) 44 3.5.6 胶体纯化 (Gel purification) 44 3.5... etds.ntut.edu.tw\|基于5个网页
3.	off-target 一种DNA靶向技术,若应用于实际的科研或医疗中,需要有高度的特异性，即要避免错误的酶切(off-target)。然而事实上，DNA剪 … baike.baidu.com\|基于3个网页

4.	EcoRI 以快速碱法抽提质粒(方法同实验一)，同时以提取的质粒作对照，用酶切（EcoRI）,电泳检测。六、注意事项 www.bioyyy.com\|基于2个网页
5.	BstE II DNA Marker www.npicp.com\|基于 1 个网页
6.	Hind III DNA Marker www.npicp.com\|基于 1 个网页

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全部全部,BamHIBamHI,Restriction enzyme cuttingRestriction enzyme cutting,off-targetoff-target

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1.

Results The result of restriction endonuclease digestion was accordance with the anticipated objective strap size .

结果酶切结果与预期目的条带大小相符；

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2.

It enables a specific gene to be located on a particular restriction enzyme fragment .

它就能使专一的基因被定位于特定的限制性内切酶切成的片段上。

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3.

Electrophoresis of the PCR products was identified , and finally PCR product was purified by gel extraction kit to prepare for digestion .

对扩增产物进行电泳鉴定，最后将 PCR 产物经胶回收试剂盒纯化，以备酶切。

4.

The difference of genetic properties among the three breeds was also significant .

三种酶切位点中，三个品种之间的遗传特性的差异也较为明显。

5.

The recombinant plasmid with the hammerhead ribozyme gene was correct by digestion identification .

核酶基因重组子经酶切鉴定序列正确。

6.

Through digestion and sequencing we identified the validity of the reorganization of the product .

通过酶切和测序鉴定重组产物的正确性。

7.

Several strains of meat-type and egg laying chickens were found to segregate for an HindIII RFLP located in the intron preceding exon 4.

几株肉型和产蛋的鸡被发现隔离的酶切多态性位于第4 外显子内含子前面。

8.

Both genes were correctly cloned and identified by PCR, restriction enzyme digestion and sequencing .

经 PCR 鉴定、酶切鉴定和测序说明所克隆的两种基因是正确的。

9.

10.

Antisense RNA of survivin was confirmed by restriction enzyme digestion and DNA sequencing .

经限制性酶切和测序鉴定所构建的反义核酸是否正确；

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