DAPI染色（原理、步骤） - 知乎
WebSep 14, 2022 · DAPI染色（原理、步骤）. biofount科研试剂. DAPI是一种可对DNA 染色的细胞核染色试剂，常用于细胞凋亡检测。. DAPI染色原理. DAPI是含有特定AT序列DNA的一种嵌入剂，它能像Hoechst染料一样粘附在DNA双螺旋的小沟区。. 尽管DAPI不能通过活细胞膜，但却能穿透扰乱的细胞 ...
DAPI (4',6-diamidino-2-phenylindole) - Thermo Fisher Scientific
WebDAPI (4′,6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits ~20-fold enhancement of fluorescence upon binding to AT regions of dsDNA. It is excited by the violet (405 nm) laser line and is commonly used as a nuclear counterstain in fluorescence microscopy, flow cytometry, and chromosome staining.
DAPI（4',6-二脒基-2苯基吲哚） | Thermo Fisher Scientific - CN
WebDAPI 由紫色（405 nm）激光激发，通常在荧光显微术、流式细胞术和染色体染色中作为核复染剂。. 由于其光谱特性，DAPI 非常适合与绿色（Invitrogen Alexa Fluor 488, FITC, GFP）和红色（Invitrogen Alexa Fluor 594，rhodamine，Invitrogen Texas red, mCherry, mKate-2）荧光基团在多色实验 ...
DAPI_百度百科
WebDAPI，即4',6-二脒基-2-苯基吲哚，是一种能够与 DNA 强力结合的 荧光 染料 ，常用于荧光 显微镜 观测。. 因为DAPI可以透过完整的 细胞膜 ，它可以用于 活细胞 和固定细胞的 染色 。. 中文名. DAPI. 外文名. 4',6-diamidino-2-phenylindole. 别 名. 4',6-二脒基-2-苯基吲哚. 分子 …
DAPI染色液(C1005) - Beyotime
WebOct 10, 2016 · DAPI染色液 (DAPI Staining Solution)是经过精心优化几乎适用于所有常见细胞和组织细胞核染色的染色液。. DAPI，即2- (4-Amidinophenyl)-6-indolecarbamidine dihydrochloride，也称DAPI dihydrochloride，分子式为C 16 H 15 N 5 ·2HCl ，分子量为350.25 ，CAS Number 28718-90-3。. DAPI是一种可以穿透细胞 ...
DAPI - 维基百科，自由的百科全书
WebDAPI即4',6-二脒基-2-苯基吲哚（4',6-diamidino-2-phenylindole），是一種能夠與DNA強力結合的螢光 染料，常用於螢光顯微鏡觀測 。因為DAPI可以透過完整的細胞膜 ，它可以用于活細胞和固定細胞的染色。
DAPI - Wikipedia
WebDAPI (pronounced 'DAPPY', /ˈdæpiː/), or 4′,6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to adenine–thymine-rich regions in DNA. It is used extensively in fluorescence microscopy .
DAPI Protocol for Fluorescence Imaging - Thermo Fisher Scientific
WebA popular nuclear and chromosome counterstain, DAPI (4′,6-diamidino-2-phenylindole) emits blue fluorescence upon binding to AT regions of DNA. This protocol can be used for: Nucleic acid (nuclear) staining in fluorescence microscopy
DAPI: a DNA-specific fluorescent probe - PubMed
WebDAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. The physicochemical properties of the dye and its complexes with nucleic acids and history of …
DAPI Counterstaining Protocols | Thermo Fisher Scientific - US
WebDAPI is a popular nuclear counterstain for use in multicolor fluorescent techniques. Its blue fluorescence stands out in vivid contrast to green, yellow, or red fluorescent probes of other structures. When used according to our protocols, DAPI stains nuclei specifically, with little or no cytoplasmic labeling.
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