Denaturing high performance liquid chromatography - Wikipedia
WebDenaturing High Performance Liquid Chromatography (DHPLC) is a method of chromatography for the detection of base substitutions, small deletions or insertions in the DNA. Thanks to its speed and high resolution, this method is particularly useful for finding polymorphisms in DNA.
Denaturing high-performance liquid chromatography: A review
WebDenaturing high-performance liquid chromatography (DHPLC) compares two or more chromosomes as a mixture of denatured and reannealed PCR amplicons, revealing the presence of a mutation by the differential retention of homo- and heteroduplex DNA on reversed-phase chromatography supports under partial denaturation.
Denaturing High Performance Liquid Chromatography
WebDenaturing high-performance liquid chromatography (dHPLC) methods are ideal for scanning genes for novel mutations and for analyzing large sample sizes at low cost. dHPLC can also be used for genotyping specific mutations or polymorphisms.
Denaturing High-Performance Liquid Chromatography Using the …
WebDenaturing high-performance liquid chromatography (DHPLC) is a chromatographic mutation analysis technique that is based on temperature-dependent separation of DNA containing mismatched base pairs from polymerase chain …
Denaturing high performance liquid chromatography (DHPLC) …
WebMar 1, 1998 · Abstract. Denaturing high performance liquid chromatography (DHPLC) has been described recently as a method for screening DNA samples for single nucleotide polymorphisms and inherited mutations. Thirty-eight DNAs, 22 of which were heterozygous for previously characterized rearranged transforming gene ( RET) or cystic fibrosis transmembrane ...
Denaturing High Pressure Liquid Chromatography (DHPLC) for …
WebDec 1, 2001 · Denaturing high pressure liquid chromatography (DHPLC) is a relatively new technique, which uses heteroduplex formation between wild-type and mutated DNA strands to identify mutations.
Denaturing High-Performance Liquid Chromatography
WebAbstract. Denaturing high performance liquid chromatography (dHPLC) is a fast and reliable technique for the DNA variation screening ( 1, 2 ).It can detect in minutes with close to 100% sensitivity and specificity single-base substitutions as well as small deletions and insertions in DNA fragments ranging from 80-1500 base pairs in size ( 3, 4 ...
Use of PCR‐DHPLC (Polymerase Chain Reaction—Denaturing High Performance ...
WebOct 9, 2012 · Use of PCR‐DHPLC (Polymerase Chain Reaction—Denaturing High Performance Liquid Chromatography) for the Rapid Differentiation of Industrial Saccharomyces pastorianus and Saccharomyces cerevisiae Strains - Hutzler - 2010 - Journal of the Institute of Brewing - Wiley Online Library.
Robust microsatellite instability (MSI) analysis by denaturing …
WebSep 12, 2003 · It took just 9 min per PCR product to determine MSI or microsatellite stability (MSS) using the new protocol. The DHPLC results were confirmed with conventional gel-based electrophoresis and ...
Denaturing High-Performance Liquid Chromatography for Mutation ...
WebDenaturing high-performance liquid chromatography (DHPLC) is an accurate and efficient screening technique used for detecting deoxyribonucleic acid sequence changes by heteroduplex analysis. It can also be used for genotyping of …
- Some results have been removed
Skip to content