85–1.6V. One possibility is that our slides, which were simple preparations consisting of a drop of the fluid, with another coverslip simply placed on top, were of significantly different thickness.
In this technique, processing is done while cells are still attached to the coverslip, and the coverslip is removed only after polymerization assuring well preserved morphology. Even individual cells ...
When viewing any slide with a microscope, a small square or circle of thin glass called a coverslip is placed over the specimen. It protects the microscope and prevents the slide from drying out ...
To investigate the disassembly of these old networks, graduate student Ekram Towsif attached actin filaments to a coverslip with the barbed ends floating free. He ran buffer containing CAP through the ...
When the cells have grown to sufficient density in the 24-well plate, treat them with trypsin and then plate them in two wells of a 6-well plate, one of which contains a coverslip. Use the cells ...
To minimize this problem, researchers commonly revert to evanescent wave excitation, but this precludes analysis of cellular regions distal from the coverslip. A clever strategy for improving the ...
The OMX imaging platform also offers TIRF (Total Internal Reflection Fluorescence) microscopy, which illuminates fluorophores within ~100 nm depth from the coverslip-sample interface without ...