Sperm concentrations >250,000/ml were detected by hemacytometer in 40 samples, 37 of which produced a positive result with the SpermCheck ® device (93% sensitivity). The concentrations of the ...
and counted respectively using a hemacytometer. The fraction of the floating cells were stained for 10 min with acridine orange (2 μg/mL) in PBS and were viewed immediately by fluorescence ...