The vast majority of peptide-centric applications incorporate trypsin and search for tryptic peptides, but other enzymes or cleavage agents can be employed. However, searching for nontryptic ...

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fragmentation approach can improve the sequence coverage for casein peptides and post-translational modification (PTM) characterization. This app note highlights the use of EAD/CID to enhance the ...

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Differing blood culture media may affect the chances of isolation of these organisms, and, though brain-heart infusion, thiol, tryptic soy, and glucose-brain infusion broths have all proved ...

Desorption electrospray ionization mass spectrometry was investigated as a method to detect and identify peptides from tryptic digests of cytochrome c and myoglobin separated on ProteoChrom HPTLC ...

Protein bands were excised with a razor from the gel or membrane. Tryptic peptides from an 80-kD Coomassie band were extracted using 50% acetonitrile and 5% formic acid. They were then dried and ...

The tryptic peptides were fractionated by high-pH, reverse-phase high-performance liquid chromatography (HPLC) using a Thermo Betasil C18 column (5 μm particle size, 10 mm I.D., 250 mm length).

This app note highlights the use of electron-activated dissociation and collision-induced dissociation to enhance the fragmentation of peptides from casein tryptic digests. This application note ...